Dataset Information

Expression profiles of miRNA-122 and its target CAT1 in minipigs (Sus scrofa) fed a high-cholesterol diet.

ABSTRACT: The Göttingen minipig is an excellent model for studying effects of dietary high-fat intake on obesity. In this study, we analyzed the expression level of microRNA-122 (miRNA-122) and its target mRNA, CAT1, in intact young male minipigs fed either high-cholesterol or standard diet for 11 wk. MiRNA-122 and CAT1 are known to be important regulators of lipid metabolism. The weight of the young minipigs was monitored once a week during the feeding period; measurements of total cholesterol, triglycerides, high-density lipoproteins, and low-density lipoproteins were recorded at 4 time points (8, 14, 16, and 19 wk of age) in fasting animals during the feeding scheme. Body weight, total cholesterol, and high-density lipoproteins were higher in pigs fed the high-cholesterol compared with the standard diet. In contrast, the level of triglycerides was lower in pigs on the high-cholesterol diet than those receiving the standard diet. Pigs fed high-cholesterol also had lower miRNA-122 levels than did those fed the standard diet. These results suggest that in our minipigs, the increase in weight and cholesterol levels resulting from subchronic (11 wk) feeding of a high-cholesterol diet is correlated with a decrease in the expression of miRNA-122, confirming the implication of this microRNA in obesity. Gene expression levels of CAT1 did not differ between groups.

SUBMITTER: Cirera S

PROVIDER: S-EPMC2855041 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature

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Expression profiles of miRNA-122 and its target CAT1 in minipigs (Sus scrofa) fed a high-cholesterol diet.

Cirera Susanna S Birck Malene M Busk Peter K PK Fredholm Merete M

Comparative medicine 20100401 2

The Göttingen minipig is an excellent model for studying effects of dietary high-fat intake on obesity. In this study, we analyzed the expression level of microRNA-122 (miRNA-122) and its target mRNA, CAT1, in intact young male minipigs fed either high-cholesterol or standard diet for 11 wk. MiRNA-122 and CAT1 are known to be important regulators of lipid metabolism. The weight of the young minipigs was monitored once a week during the feeding period; measurements of total cholesterol, triglycer ...[more]

PMID: 20412689

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Dataset Information

Expression profiles of miRNA-122 and its target CAT1 in minipigs (Sus scrofa) fed a high-cholesterol diet.

Publications

Expression profiles of miRNA-122 and its target CAT1 in minipigs (Sus scrofa) fed a high-cholesterol diet.

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