Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.
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ABSTRACT:
SUBMITTER: Pohjoismaki JL
PROVIDER: S-EPMC2857715 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Publications
Mammalian mitochondrial DNA replication intermediates are essentially duplex but contain extensive tracts of RNA/DNA hybrid.
Pohjoismäki Jaakko L O JL Holmes J Bradley JB Wood Stuart R SR Yang Ming-Yao MY Yasukawa Takehiro T Reyes Aurelio A Bailey Laura J LJ Cluett Tricia J TJ Goffart Steffi S Willcox Smaranda S Rigby Rachel E RE Jackson Andrew P AP Spelbrink Johannes N JN Griffith Jack D JD Crouch Robert J RJ Jacobs Howard T HT Holt Ian J IJ
Journal of molecular biology 20100223 5
We demonstrate, using transmission electron microscopy and immunopurification with an antibody specific for RNA/DNA hybrid, that intact mitochondrial DNA replication intermediates are essentially duplex throughout their length but contain extensive RNA tracts on one strand. However, the extent of preservation of RNA in such molecules is highly dependent on the preparative method used. These findings strongly support the strand-coupled model of mitochondrial DNA replication involving RNA incorpor ...[more]
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