Ontology highlight
ABSTRACT:
SUBMITTER: Winter MB
PROVIDER: S-EPMC2859244 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Winter Michael B MB McLaurin Emily J EJ Reece Steven Y SY Olea Charles C Nocera Daniel G DG Marletta Michael A MA
Journal of the American Chemical Society 20100401 16
Hemoprotein-based scaffolds containing phosphorescent ruthenium(II) CO mesoporphyrin IX (RuMP) are reported here for oxygen (O(2)) sensing in biological contexts. RuMP was incorporated into the protein scaffolds during protein expression utilizing a novel method that we have described previously. A high-resolution (2.00 A) crystal structure revealed that the unnatural porphyrin binds to the proteins in a manner similar to the native heme and does not perturb the protein fold. The protein scaffol ...[more]