Ontology highlight
ABSTRACT:
SUBMITTER: Hollands K
PROVIDER: S-EPMC2859248 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Hollands Kerry K Lee David J DJ Lloyd Georgina S GS Busby Stephen J W SJ
Molecular microbiology 20091015 5
The Escherichia coli aer regulatory region contains a single promoter that is recognized by RNA polymerase containing the flagellar sigma factor, sigma(28). Expression from this promoter is dependent on direct activation by the cyclic AMP receptor protein, which binds to a target centred 49.5 base pairs upstream from the transcript start. Activator-dependent transcription from the aer promoter was reconstituted in vitro, and a tethered inorganic nuclease was used to find the position of the C-te ...[more]