Ontology highlight
ABSTRACT:
SUBMITTER: Dreosti E
PROVIDER: S-EPMC2859341 | biostudies-literature | 2009 Dec
REPOSITORIES: biostudies-literature
Dreosti Elena E Odermatt Benjamin B Dorostkar Mario M MM Lagnado Leon L
Nature methods 20091108 12
To image synaptic activity within neural circuits, we tethered the genetically encoded calcium indicator (GECI) GCaMP2 to synaptic vesicles by fusion to synaptophysin. The resulting reporter, SyGCaMP2, detected the electrical activity of neurons with two advantages over existing cytoplasmic GECIs: it identified the locations of synapses and had a linear response over a wider range of spike frequencies. Simulations and experimental measurements indicated that linearity arises because SyGCaMP2 sam ...[more]