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High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis.

ABSTRACT: Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.

SUBMITTER: Laremore TN

PROVIDER: S-EPMC2860026 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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High-resolution preparative separation of glycosaminoglycan oligosaccharides by polyacrylamide gel electrophoresis.

Laremore Tatiana N TN Ly Mellisa M Solakyildirim Kemal K Zagorevski Dmitri V DV Linhardt Robert J RJ

Analytical biochemistry 20100306 2

Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spec ...[more]

PMID: 20211145

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