Ontology highlight
ABSTRACT:
SUBMITTER: van den Berg DL
PROVIDER: S-EPMC2860243 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
van den Berg Debbie L C DLC Snoek Tim T Mullin Nick P NP Yates Adam A Bezstarosti Karel K Demmers Jeroen J Chambers Ian I Poot Raymond A RA
Cell stem cell 20100401 4
Transcription factors, such as Oct4, are critical for establishing and maintaining pluripotent cell identity. Whereas the genomic locations of several pluripotency transcription factors have been reported, the spectrum of their interaction partners is underexplored. Here, we use an improved affinity protocol to purify Oct4-interacting proteins from mouse embryonic stem cells (ESCs). Subsequent purification of Oct4 partners Sall4, Tcfcp2l1, Dax1, and Esrrb resulted in an Oct4 interactome of 166 p ...[more]