Ontology highlight
ABSTRACT:
SUBMITTER: Gu H
PROVIDER: S-EPMC2860480 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Gu Hongcang H Bock Christoph C Mikkelsen Tarjei S TS Jäger Natalie N Smith Zachary D ZD Tomazou Eleni E Gnirke Andreas A Lander Eric S ES Meissner Alexander A
Nature methods 20100110 2
Bisulfite sequencing measures absolute levels of DNA methylation at single-nucleotide resolution, providing a robust platform for molecular diagnostics. We optimized bisulfite sequencing for genome-scale analysis of clinical samples: here we outline how restriction digestion targets bisulfite sequencing to hotspots of epigenetic regulation and describe a statistical method for assessing significance of altered DNA methylation patterns. Thirty nanograms of DNA was sufficient for genome-scale anal ...[more]