Dataset Information

Global microRNA expression profiles in insulin target tissues in a spontaneous rat model of type 2 diabetes.

ABSTRACT:

Aims/hypothesis

MicroRNAs regulate a broad range of biological mechanisms. To investigate the relationship between microRNA expression and type 2 diabetes, we compared global microRNA expression in insulin target tissues from three inbred rat strains that differ in diabetes susceptibility.

Methods

Using microarrays, we measured the expression of 283 microRNAs in adipose, liver and muscle tissue from hyperglycaemic (Goto-Kakizaki), intermediate glycaemic (Wistar Kyoto) and normoglycaemic (Brown Norway) rats (n = 5 for each strain). Expression was compared across strains and validated using quantitative RT-PCR. Furthermore, microRNA expression variation in adipose tissue was investigated in 3T3-L1 adipocytes exposed to hyperglycaemic conditions.

Results

We found 29 significantly differentiated microRNAs (p(adjusted) < 0.05): nine in adipose tissue, 18 in liver and two in muscle. Of these, five microRNAs had expression patterns that correlated with the strain-specific glycaemic phenotype. MiR-222 (p(adjusted) = 0.0005) and miR-27a (p(adjusted) = 0.006) were upregulated in adipose tissue; miR-195 (p(adjusted) = 0.006) and miR-103 (p(adjusted) = 0.04) were upregulated in liver; and miR-10b (p(adjusted) = 0.004) was downregulated in muscle. Exposure of 3T3-L1 adipocytes to increased glucose concentration upregulated the expression of miR-222 (p = 0.008), miR-27a (p = 0.02) and the previously reported miR-29a (p = 0.02). Predicted target genes of these differentially expressed microRNAs are involved in pathways relevant to type 2 diabetes.

Conclusion

The expression patterns of miR-222, miR-27a, miR-195, miR-103 and miR-10b varied with hyperglycaemia, suggesting a role for these microRNAs in the pathophysiology of type 2 diabetes, as modelled by the Gyoto-Kakizaki rat. We observed similar patterns of expression of miR-222, miR-27a and miR-29a in adipocytes as a response to increased glucose levels, which supports our hypothesis that altered expression of microRNAs accompanies primary events related to the pathogenesis of type 2 diabetes.

SUBMITTER: Herrera BM

PROVIDER: S-EPMC2860560 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Publications

Global microRNA expression profiles in insulin target tissues in a spontaneous rat model of type 2 diabetes.

Herrera B M BM Lockstone H E HE Taylor J M JM Ria M M Barrett A A Collins S S Kaisaki P P Argoud K K Fernandez C C Travers M E ME Grew J P JP Randall J C JC Gloyn A L AL Gauguier D D McCarthy M I MI Lindgren C M CM

Diabetologia 20100303 6

<h4>Aims/hypothesis</h4>MicroRNAs regulate a broad range of biological mechanisms. To investigate the relationship between microRNA expression and type 2 diabetes, we compared global microRNA expression in insulin target tissues from three inbred rat strains that differ in diabetes susceptibility.<h4>Methods</h4>Using microarrays, we measured the expression of 283 microRNAs in adipose, liver and muscle tissue from hyperglycaemic (Goto-Kakizaki), intermediate glycaemic (Wistar Kyoto) and normogly ...[more]

PMID: 20198361

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Dataset Information

Global microRNA expression profiles in insulin target tissues in a spontaneous rat model of type 2 diabetes.

Aims/hypothesis

Methods

Results

Conclusion

Publications

Global microRNA expression profiles in insulin target tissues in a spontaneous rat model of type 2 diabetes.

Similar Datasets

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