Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
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ABSTRACT:
SUBMITTER: Leischner U
PROVIDER: S-EPMC2861007 | biostudies-literature | 2010
REPOSITORIES: biostudies-literature
Publications
Formalin-induced fluorescence reveals cell shape and morphology in biological tissue samples.
Leischner Ulrich U Schierloh Anja A Zieglgänsberger Walter W Dodt Hans-Ulrich HU
PloS one 20100428 4
Ultramicroscopy is a powerful tool to reveal detailed three-dimensional structures of large microscopical objects. Using high magnification, we observed that formalin induces fluorescence more in extra-cellular space and stains cellular structures negatively, rendering cells as dark objects in front of a bright background. Here, we show this effect on a three-dimensional image stack of a hippocampus sample, focusing on the CA1 region. This method, called FIF-Ultramicroscopy, allows for the three ...[more]
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