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Measurement of recent exposure to Phlebotomus argentipes, the vector of Indian visceral Leishmaniasis, by using human antibody responses to sand fly saliva.


ABSTRACT: Antibody (IgG) responses to the saliva of Phlebotomus argentipes were investigated using serum samples from regions of India endemic and non-endemic for visceral leishmaniasis (VL). By pre-adsorbing the sera against the saliva of the competing human-biting but non-VL vector P. papatasi, we significantly improved the specificity of a P. argentipes saliva enzyme-linked immunosorbent assay. Using this method, we observed a statistically significant correlation between antibodies to P. argenitpes saliva and the average indoor density of female sand flies. Additionally, the method was able to detect recent changes in vector exposure when sera from VL patients were assayed before, during, and after hospitalization and protected from sand fly bites under untreated bed nets. Collectively, these results highlight the utility of antibodies to P. argentipes saliva as an important tool to evaluate VL vector control programs.

SUBMITTER: Clements MF 

PROVIDER: S-EPMC2861389 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Measurement of recent exposure to Phlebotomus argentipes, the vector of Indian visceral Leishmaniasis, by using human antibody responses to sand fly saliva.

Clements Meredith F MF   Gidwani Kamlesh K   Kumar Rajiv R   Hostomska Jitka J   Dinesh Diwakar S DS   Kumar Vijay V   Das Pradeep P   Müller Ingrid I   Hamilton Gordon G   Volfova Vera V   Boelaert Marleen M   Das Murari M   Rijal Suman S   Picado Albert A   Volf Petr P   Sundar Shyam S   Davies Clive R CR   Rogers Matthew E ME  

The American journal of tropical medicine and hygiene 20100501 5


Antibody (IgG) responses to the saliva of Phlebotomus argentipes were investigated using serum samples from regions of India endemic and non-endemic for visceral leishmaniasis (VL). By pre-adsorbing the sera against the saliva of the competing human-biting but non-VL vector P. papatasi, we significantly improved the specificity of a P. argentipes saliva enzyme-linked immunosorbent assay. Using this method, we observed a statistically significant correlation between antibodies to P. argenitpes sa  ...[more]

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