FRT-seq: amplification-free, strand-specific transcriptome sequencing.
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ABSTRACT:
SUBMITTER: Mamanova L
PROVIDER: S-EPMC2861772 | biostudies-literature | 2010 Feb
REPOSITORIES: biostudies-literature
Publications
FRT-seq: amplification-free, strand-specific transcriptome sequencing.
Mamanova Lira L Andrews Robert M RM James Keith D KD Sheridan Elizabeth M EM Ellis Peter D PD Langford Cordelia F CF Ost Tobias W B TW Collins John E JE Turner Daniel J DJ
Nature methods 20100117 2
We report an alternative approach to transcriptome sequencing for the Illumina Genome Analyzer, in which the reverse transcription reaction takes place on the flowcell. No amplification is performed during the library preparation, so PCR biases and duplicates are avoided, and because the template is poly(A)(+) RNA rather than cDNA, the resulting sequences are necessarily strand-specific. The method is compatible with paired- or single-end sequencing. ...[more]
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