Insights into heterocyclization from two highly similar enzymes.
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ABSTRACT:
SUBMITTER: McIntosh JA
PROVIDER: S-EPMC2862276 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Publications
Insights into heterocyclization from two highly similar enzymes.
McIntosh John A JA Donia Mohamed S MS Schmidt Eric W EW
Journal of the American Chemical Society 20100301 12
The cyanobactin biosynthetic pathways pat and tru, isolated from metagenomes of marine animals, lead to diverse natural products containing heterocycles derived from Cys, Ser, and Thr. Previous work has shown that PatD and TruD are extremely broad-substrate heterocyclase enzymes. These enzymes are virtually identical in their N-terminal putative catalytic domains, but only approximately 77% identical in their C-terminal putative substrate-binding domains. Here, we show that these differences all ...[more]
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