Ontology highlight
ABSTRACT:
SUBMITTER: Fu D
PROVIDER: S-EPMC2863699 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Fu Dragony D Brophy Jennifer A N JA Chan Clement T Y CT Atmore Kyle A KA Begley Ulrike U Paules Richard S RS Dedon Peter C PC Begley Thomas J TJ Samson Leona D LD
Molecular and cellular biology 20100322 10
tRNA nucleosides are extensively modified to ensure their proper function in translation. However, many of the enzymes responsible for tRNA modifications in mammals await identification. Here, we show that human AlkB homolog 8 (ABH8) catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine (mcm(5)U) at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival. We find that ABH8 interacts specifically with tRNAs containing mcm(5)U and t ...[more]