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Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR.

ABSTRACT: The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h.

SUBMITTER: Hindiyeh M

PROVIDER: S-EPMC2863859 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Rapid detection of influenza A pandemic (H1N1) 2009 virus neuraminidase resistance mutation H275Y by real-time reverse transcriptase PCR.

Hindiyeh Musa M Ram Daniela D Mandelboim Michal M Meningher Tal T Hirsh Shira S Robinov Jana J Levy Virginia V Orzitzer Sara S Azar Roberto R Grossman Zehava Z Mendelson Ella E

Journal of clinical microbiology 20100310 5

The emergence of oseltamivir-resistant influenza A pandemic (H1N1) 2009 virus highlights the need for rapid oseltamivir resistance screening. We report the development and validation of high-throughput real-time reverse transcriptase PCR assays for the detection of the H275Y substitution in the neuraminidase 1 gene that can be accomplished in 3 to 4 h. ...[more]

PMID: 20220153

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