Ontology highlight
ABSTRACT:
SUBMITTER: Kim CM
PROVIDER: S-EPMC2863927 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Kim Cheol Min CM Song Eun Sil ES Jang Hyun Jung HJ Kim Hyun-Ju HJ Lee Sangyeop S Shin Jeong Hwan JH Kim Sun Joo SJ Jeong Seok Hoon SH Jeong Joseph J Koh Kwangnak K Choi Go Eun GE Lee Eun Yup EY Chang Chulhun L CL
Journal of clinical microbiology 20100317 5
Oligonucleotide chips targeting the bacterial internal transcribed spacer region (ITS) of the 16S-23S rRNA gene, which contains genus- and species-specific regions, were developed and evaluated. Forty-three sequences were designed consisting of 1 universal, 3 Gram stain-specific, 9 genus-specific, and 30 species-specific probes. The specificity of the probes was confirmed using bacterial type strains including 54 of 52 species belonging to 18 genera. The performance of the probes was evaluated u ...[more]