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Mechanism of eIF6-mediated inhibition of ribosomal subunit joining.

ABSTRACT: During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.

SUBMITTER: Gartmann M

PROVIDER: S-EPMC2865328 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Mechanism of eIF6-mediated inhibition of ribosomal subunit joining.

Gartmann Marco M Blau Michael M Armache Jean-Paul JP Mielke Thorsten T Topf Maya M Beckmann Roland R

The Journal of biological chemistry 20100331 20

During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escheric ...[more]

PMID: 20356839

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