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De novo structure generation using chemical shifts for proteins with high-sequence identity but different folds.

ABSTRACT: Proteins with high-sequence identity but very different folds present a special challenge to sequence-based protein structure prediction methods. In particular, a 56-residue three-helical bundle protein (GA(95)) and an alpha/beta-fold protein (GB(95)), which share 95% sequence identity, were targets in the CASP-8 structure prediction contest. With only 12 out of 300 submitted server-CASP8 models for GA(95) exhibiting the correct fold, this protein proved particularly challenging despite its small size. Here, we demonstrate that the information contained in NMR chemical shifts can readily be exploited by the CS-Rosetta structure prediction program and yields adequate convergence, even when input chemical shifts are limited to just amide (1)H(N) and (15)N or (1)H(N) and (1)H(alpha) values.

SUBMITTER: Shen Y

PROVIDER: S-EPMC2865713 | biostudies-literature | 2010 Feb

REPOSITORIES: biostudies-literature

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De novo structure generation using chemical shifts for proteins with high-sequence identity but different folds.

Shen Yang Y Bryan Philip N PN He Yanan Y Orban John J Baker David D Bax Ad A

Protein science : a publication of the Protein Society 20100201 2

Proteins with high-sequence identity but very different folds present a special challenge to sequence-based protein structure prediction methods. In particular, a 56-residue three-helical bundle protein (GA(95)) and an alpha/beta-fold protein (GB(95)), which share 95% sequence identity, were targets in the CASP-8 structure prediction contest. With only 12 out of 300 submitted server-CASP8 models for GA(95) exhibiting the correct fold, this protein proved particularly challenging despite its smal ...[more]

PMID: 19998407

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