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Apoptosis-inducing galactolipids from a cultured marine diatom, Phaeodactylum tricornutum.

ABSTRACT: Two monogalactosyl diacylglycerols, 1 and 2, were isolated from the marine diatom Phaeodactylum tricornutum, using the patented ApopScreen cell-based screen for apoptosis-inducing, potential anticancer compounds. The molecular structures of the galactolipids were determined using a combination of NMR, mass spectrometry, and chemical degradation. The bioactivities were confirmed using a specific apoptosis induction assay based on genetically engineered mammalian cell lines with differential, defined capacities for apoptosis. The galactolipids induce apoptosis in micromolar concentrations. This is the first report of apoptosis induction by galactolipids.

SUBMITTER: Andrianasolo EH

PROVIDER: S-EPMC2866075 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Apoptosis-inducing galactolipids from a cultured marine diatom, Phaeodactylum tricornutum.

Andrianasolo Eric H EH Haramaty Liti L Vardi Assaf A White Eileen E Lutz Richard R Falkowski Paul P

Journal of natural products 20080621 7

Two monogalactosyl diacylglycerols, 1 and 2, were isolated from the marine diatom Phaeodactylum tricornutum, using the patented ApopScreen cell-based screen for apoptosis-inducing, potential anticancer compounds. The molecular structures of the galactolipids were determined using a combination of NMR, mass spectrometry, and chemical degradation. The bioactivities were confirmed using a specific apoptosis induction assay based on genetically engineered mammalian cell lines with differential, defi ...[more]

PMID: 18570469

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