Ontology highlight
ABSTRACT:
SUBMITTER: Bai L
PROVIDER: S-EPMC2867244 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Bai Lu L Charvin Gilles G Siggia Eric D ED Cross Frederick R FR
Developmental cell 20100401 4
Many promoters in eukaryotes have nucleosome-depleted regions (NDRs) containing transcription factor binding sites. However, the functional significance of NDRs is not well understood. Here, we examine NDR function in two cell cycle-regulated promoters, CLN2pr and HOpr, by varying nucleosomal coverage of the binding sites of their activator, Swi4/Swi6 cell-cycle box (SCB)-binding factor (SBF), and probing the corresponding transcriptional activity in individual cells with time-lapse microscopy. ...[more]