Ontology highlight
ABSTRACT:
SUBMITTER: Gracz AD
PROVIDER: S-EPMC2867430 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Gracz Adam D AD Ramalingam Sendhilnathan S Magness Scott T ST
American journal of physiology. Gastrointestinal and liver physiology 20100225 5
The inability to identify, isolate, and culture intestinal epithelial stem cells (IESCs) has been prohibitive to the study and therapeutic utilization of these cells. Using a Sox9(EGFP) mouse model, we demonstrate that Sox9(EGFP) fluorescence signatures can be used to differentiate between and enrich for progenitors (Sox9(EGFPsubLo)) and multipotent IESCs (Sox9(EGFPlo)). Sox9(EGFPlo) cells generate "organoids" in a recently defined culture system that mimics the native IESC niche. These organoid ...[more]