Ontology highlight
ABSTRACT:
SUBMITTER: Guttman M
PROVIDER: S-EPMC2868100 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Guttman Mitchell M Garber Manuel M Levin Joshua Z JZ Donaghey Julie J Robinson James J Adiconis Xian X Fan Lin L Koziol Magdalena J MJ Gnirke Andreas A Nusbaum Chad C Rinn John L JL Lander Eric S ES Regev Aviv A
Nature biotechnology 20100502 5
Massively parallel cDNA sequencing (RNA-Seq) provides an unbiased way to study a transcriptome, including both coding and noncoding genes. Until now, most RNA-Seq studies have depended crucially on existing annotations and thus focused on expression levels and variation in known transcripts. Here, we present Scripture, a method to reconstruct the transcriptome of a mammalian cell using only RNA-Seq reads and the genome sequence. We applied it to mouse embryonic stem cells, neuronal precursor cel ...[more]