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Solution structure of the N-terminal domain of DC-UbP/UBTD2 and its interaction with ubiquitin.

ABSTRACT: DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. The solution structure and backbone dynamics of the C-terminal Ub-like (UbL) domain were elucidated in our previous work. To further understand the biological function of DC-UbP, we then solved the solution structure of the N-terminal domain of DC-UbP (DC-UbP_N) and studied its Ub binding properties by NMR techniques. The results show that DC-UbP_N holds a novel structural fold and acts as a Ub-binding domain (UBD) but with low affinity. This implies that the DC-UbP protein, composing of a combination of both UbL and UBD domains, might play an important role in regulating protein ubiquitination and delivery of ubiquitinated substrates in eukaryotic cells.

SUBMITTER: Song AX

PROVIDER: S-EPMC2868252 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Solution structure of the N-terminal domain of DC-UbP/UBTD2 and its interaction with ubiquitin.

Song Ai-Xin AX Zhou Chen-Jie CJ Guan Xiao X Sze Kong-Hung KH Hu Hong-Yu HY

Protein science : a publication of the Protein Society 20100501 5

DC-UbP/UBTD2 is a ubiquitin (Ub) domain-containing protein first identified from dendritic cells, and is implicated in ubiquitination pathway. The solution structure and backbone dynamics of the C-terminal Ub-like (UbL) domain were elucidated in our previous work. To further understand the biological function of DC-UbP, we then solved the solution structure of the N-terminal domain of DC-UbP (DC-UbP_N) and studied its Ub binding properties by NMR techniques. The results show that DC-UbP_N holds ...[more]

PMID: 20440844

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