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Effects of environmental stress on stability of tandem repeats in Escherichia coli O157:H7.


ABSTRACT: Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication but changed after exposure to irradiation, elevated temperatures, and starvation conditions. The pO157 plasmid was frequently lost under these stress conditions. Environmental stresses may increase phylogenetic diversity as measured by MLVA.

SUBMITTER: Cooley MB 

PROVIDER: S-EPMC2869130 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature

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Effects of environmental stress on stability of tandem repeats in Escherichia coli O157:H7.

Cooley Michael B MB   Carychao Diana D   Nguyen Kimberly K   Whitehand Linda L   Mandrell Robert R  

Applied and environmental microbiology 20100326 10


Multilocus variable-number tandem-repeat analysis (MLVA) is used for source tracking Escherichia coli O157:H7 in agricultural environments. Tandem repeats were stable after limited replication but changed after exposure to irradiation, elevated temperatures, and starvation conditions. The pO157 plasmid was frequently lost under these stress conditions. Environmental stresses may increase phylogenetic diversity as measured by MLVA. ...[more]

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