Ontology highlight
ABSTRACT:
SUBMITTER: Perriman R
PROVIDER: S-EPMC2872779 | biostudies-literature | 2010 May
REPOSITORIES: biostudies-literature
Perriman Rhonda R Ares Manuel M
Molecular cell 20100501 3
U2 snRNA-intron branchpoint pairing is a critical step in pre-mRNA recognition by the splicing apparatus, but the mechanism by which these two RNAs engage each other is unknown. Here, we identify a U2 snRNA structure, the branchpoint-interacting stem loop (BSL), which presents the U2 nucleotides that will contact the intron. We provide evidence that the BSL forms prior to interaction with the intron and is disrupted by the DExD/H protein Prp5p during engagement of the snRNA with the intron. In v ...[more]