Ontology highlight
ABSTRACT:
SUBMITTER: Tiemann K
PROVIDER: S-EPMC2874179 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
RNA (New York, N.Y.) 20100421 6
We have developed an algorithm for the prediction of dual-targeting short interfering RNAs (siRNAs) in which both strands are deliberately designed to separately target different mRNA transcripts with complete complementarity. An advantage of this approach versus the use of two separate duplexes is that only two strands, as opposed to four, are competing for entry into the RNA-induced silencing complex. We chose to design our dual-targeting siRNAs as Dicer substrate 25/27mer siRNAs, since design ...[more]