Chemical aminoacylation of tRNAs with fluorinated amino acids for in vitro protein mutagenesis.

ABSTRACT: This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of discrete fluorines' interaction with canonical amino acids in a native protein environment and to enable the design of fluorinated proteins with arbitrary desired properties.

SUBMITTER: Ye S 

PROVIDER: S-EPMC2874332 | biostudies-literature | 2010 Apr

REPOSITORIES: biostudies-literature