Ontology highlight
ABSTRACT:
SUBMITTER: Ye S
PROVIDER: S-EPMC2874332 | biostudies-literature | 2010 Apr
REPOSITORIES: biostudies-literature
Beilstein journal of organic chemistry 20100420
This article describes the chemical aminoacylation of the yeast phenylalanine suppressor tRNA with a series of amino acids bearing fluorinated side chains via the hybrid dinucleotide pdCpA and ligation to the corresponding truncated tRNA species. Aminoacyl-tRNAs can be used to synthesize biologically relevant proteins which contain fluorinated amino acids at specific sites by means of a cell-free translation system. Such engineered proteins are expected to contribute to our understanding of disc ...[more]