Massively parallel single-molecule manipulation using centrifugal force.

ABSTRACT: Precise manipulation of single molecules has already led to remarkable insights in physics, chemistry, biology, and medicine. However, widespread adoption of single-molecule techniques has been impeded by equipment cost and the laborious nature of making measurements one molecule at a time. We have solved these issues by developing an approach that enables massively parallel single-molecule force measurements using centrifugal force. This approach is realized in an instrument that we call the centrifuge force microscope in which objects in an orbiting sample are subjected to a calibration-free, macroscopically uniform force-field while their micro-to-nanoscopic motions are observed. We demonstrate high-throughput single-molecule force spectroscopy with this technique by performing thousand

SUBMITTER: Halvorsen K 

PROVIDER: S-EPMC2877324 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature