Ontology highlight
ABSTRACT:
SUBMITTER: Arakaki TL
PROVIDER: S-EPMC2877593 | biostudies-literature | 2008 Apr
REPOSITORIES: biostudies-literature
Arakaki Tracy L TL Buckner Frederick S FS Gillespie J Robert JR Malmquist Nicholas A NA Phillips Margaret A MA Kalyuzhniy Oleksandr O Luft Joseph R JR Detitta George T GT Verlinde Christophe L M J CL Van Voorhis Wesley C WC Hol Wim G J WG Merritt Ethan A EA
Molecular microbiology 20080226 1
Nucleotide biosynthesis pathways have been reported to be essential in some protozoan pathogens. Hence, we evaluated the essentiality of one enzyme in the pyrimidine biosynthetic pathway, dihydroorotate dehydrogenase (DHODH) from the eukaryotic parasite Trypanosoma brucei through gene knockdown studies. RNAi knockdown of DHODH expression in bloodstream form T. brucei did not inhibit growth in normal medium, but profoundly retarded growth in pyrimidine-depleted media or in the presence of the kno ...[more]