In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.
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ABSTRACT:
SUBMITTER: Perentes JY
PROVIDER: S-EPMC2877598 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Publications
In vivo imaging of extracellular matrix remodeling by tumor-associated fibroblasts.
Perentes Jean Y JY McKee Trevor D TD Ley Carsten D CD Mathiew Hannah H Dawson Michelle M Padera Timothy P TP Munn Lance L LL Jain Rakesh K RK Boucher Yves Y
Nature methods 20090118 2
Here we integrated multiphoton laser scanning microscopy and the registration of second harmonic generation images of collagen fibers to overcome difficulties in tracking stromal cell-matrix interactions for several days in live mice. We show that the matrix-modifying hormone relaxin increased tumor-associated fibroblast (TAF) interaction with collagen fibers by stimulating beta1-integrin activity, which is necessary for fiber remodeling by matrix metalloproteinases. ...[more]
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