Differential expression of genes identified by suppression subtractive hybridization in petals of opening carnation flowers.

ABSTRACT: Flower opening is an event accompanied by morphological changes in petals which include elongation, expansion, and outward-curving. Petal cell growth is a fundamental process that underlies such phenomena, but its molecular mechanism remains largely unknown. Suppression subtractive hybridization was performed between petals during the early elongation period (stage 1) and during the opening period (stage 5) in carnation flowers and a pair of subtraction libraries abundant in differentially expressed genes was constructed at each stage. 393 cDNA clones picked up by differential screening out of 1728 clones were sequenced and 235 different cDNA fragments were identified, among which 211 did not match any known nucleotide sequence of carnation genes in the databases. BLASTX search of nucleoti

SUBMITTER: Harada T 

PROVIDER: S-EPMC2877890 | biostudies-literature | 2010 May

REPOSITORIES: biostudies-literature