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Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.

ABSTRACT: Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

SUBMITTER: Luchini A

PROVIDER: S-EPMC2877922 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature

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Smart hydrogel particles: biomarker harvesting: one-step affinity purification, size exclusion, and protection against degradation.

Luchini Alessandra A Geho David H DH Bishop Barney B Tran Duy D Xia Cassandra C Dufour Robert L RL Jones Clinton D CD Espina Virginia V Patanarut Alexis A Zhou Weidong W Ross Mark M MM Tessitore Alessandra A Petricoin Emanuel F EF Liotta Lance A LA

Nano letters 20071213 1

Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The cap ...[more]

PMID: 18076201

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