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Targeted gene deletions in C. elegans using transposon excision.

ABSTRACT: We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker.

SUBMITTER: Frokjaer-Jensen C

PROVIDER: S-EPMC2878396 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Targeted gene deletions in C. elegans using transposon excision.

Frøkjaer-Jensen Christian C Davis M Wayne MW Hollopeter Gunther G Taylor Jon J Harris Todd W TW Nix Paola P Lofgren Rachel R Prestgard-Duke Michael M Bastiani Michael M Moerman Donald G DG Jorgensen Erik M EM

Nature methods 20100425 6

We developed a method, MosDEL, to generate targeted knockouts of genes in Caenorhabditis elegans by injection. We generated a double-strand break by mobilizing a Mos1 transposon adjacent to the region to be deleted; the double-stranded break is repaired using injected DNA as a template. Repair can delete up to 25 kb of DNA and simultaneously insert a positive selection marker. ...[more]

PMID: 20418868

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