Ontology highlight
ABSTRACT:
SUBMITTER: Westerman KA
PROVIDER: S-EPMC2878880 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Westerman Karen A KA Penvose Ashley A Yang Zhong Z Allen Paul D PD Vacanti Charles A CA
Experimental cell research 20100408 12
The effectiveness of cell-based therapy to treat muscle disease has been hampered by difficulties in isolating, maintaining and propagating the stem cells that are needed for treatment. Here we report the isolation of muscle-derived stem cells from both young and old mice and their propagation over extended periods of time in culture as "free-floating" myospheres. Analysis of these sphere-forming cells showed that they express stem cell antigen-1 (Sca-1), beta1 integrin (CD29), Thy-1 (CD90), and ...[more]