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Chapter 4. Predicting and characterizing protein functions through matching geometric and evolutionary patterns of binding surfaces.


ABSTRACT: Predicting protein functions from structures is an important and challenging task. Although proteins are often thought to be packed as tightly as solids, closer examination based on geometric computation reveals that they contain numerous voids and pockets. Most of them are of random nature, but some are binding sites providing surfaces to interact with other molecules. A promising approach for function prediction is to infer functions through discovery of similarity in local binding pockets, as proteins binding to similar substrates/ligands and carrying out similar functions have similar physical constraints for binding and reactions. In this chapter, we describe computational methods to distinguish those surface pockets that are likely to be involved in important biological functions, and methods to identify key residues in these pockets. We further describe how to predict protein functions at large scale from structures by detecting binding surfaces similar in residue make-ups, shape, and orientation. We also describe a Bayesian Monte Carlo method that can separate selection pressure due to biological function from pressure due to protein folding. We show how this method can be used to reconstruct the evolutionary history of binding surfaces for detecting similar binding surfaces. In addition, we briefly discuss how the negative image of a binding pocket can be casted, and how such information can be used to facilitate drug discovery.

SUBMITTER: Liang J 

PROVIDER: S-EPMC2882714 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature

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Chapter 4. Predicting and characterizing protein functions through matching geometric and evolutionary patterns of binding surfaces.

Liang Jie J   Tseng Yan-Yuan YY   Dundas Joseph J   Binkowski T Andrew TA   Joachimiak Andrzej A   Ouyang Zheng Z   Adamian Larisa L  

Advances in protein chemistry and structural biology 20080101


Predicting protein functions from structures is an important and challenging task. Although proteins are often thought to be packed as tightly as solids, closer examination based on geometric computation reveals that they contain numerous voids and pockets. Most of them are of random nature, but some are binding sites providing surfaces to interact with other molecules. A promising approach for function prediction is to infer functions through discovery of similarity in local binding pockets, as  ...[more]

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