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Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.

ABSTRACT: Several amino acids and their derivatives are prominent additives in the field of protein chemistry. This study reports the use of charged amino acids and glycine ethyl ester as precipitants in protein crystallization, using hen egg-white lysozyme (HEWL) as a model. A discussion of the crystallization of HEWL using these reagents as precipitating agents is given.

SUBMITTER: Ito L

PROVIDER: S-EPMC2882786 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Amino acids and glycine ethyl ester as new crystallization reagents for lysozyme.

Ito Len L Shiraki Kentaro K Yamaguchi Hiroshi H

Acta crystallographica. Section F, Structural biology and crystallization communications 20100527 Pt 6

Several amino acids and their derivatives are prominent additives in the field of protein chemistry. This study reports the use of charged amino acids and glycine ethyl ester as precipitants in protein crystallization, using hen egg-white lysozyme (HEWL) as a model. A discussion of the crystallization of HEWL using these reagents as precipitating agents is given. ...[more]

PMID: 20516616

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