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Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays.

ABSTRACT: We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward parallelization to large nanopore arrays.

SUBMITTER: McNally B

PROVIDER: S-EPMC2883017 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Optical recognition of converted DNA nucleotides for single-molecule DNA sequencing using nanopore arrays.

McNally Ben B Singer Alon A Yu Zhiliang Z Sun Yingjie Y Weng Zhiping Z Meller Amit A

Nano letters 20100601 6

We demonstrate the feasibility of a nanopore based single-molecule DNA sequencing method, which employs multicolor readout. Target DNA is converted according to a binary code, which is recognized by molecular beacons with two types of fluorophores. Solid-state nanopores are then used to sequentially strip off the beacons, leading to a series of detectable photon bursts, at high speed. We show that signals from multiple nanopores can be detected simultaneously, allowing straightforward paralleliz ...[more]

PMID: 20459065

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