Unknown

Dataset Information

0

Molecular hallmarks of anti-chromatin antibodies associated with the lupus susceptibility locus, Sle1.


ABSTRACT: Anti-nuclear antibodies constitute the hallmark of lupus. The NZM2410-derived Sle1 lupus susceptibility interval on murine chromosome 1 breaches tolerance, leading to the emergence of anti-nuclear autoantibodies targeting nucleosomes. However, little is known about the molecular structure of the anti-nucleosome autoantibodies from this genetically simplified mouse model of lupus. In this study, the immunoglobulin heavy chain and light chain sequences of 50 anti-nuclear monoclonal antibodies derived from five B6.Sle1(z) mice were compared to non-nuclear antibody controls. Compared to two different sets of non-nuclear antibodies, anti-nucleosome antibodies derived from B6.Sle1(z) congenic mice exhibited a high degree of clonal expansion and three distinct sequence motifs in their heavy chains - cationic CDR3 stretches, non-anionic CDR2 regions, and an increased frequency of aspartate residues at H50, which together increased the likelihood of an antibody being chromatin-reactive by approximately 4-fold.

SUBMITTER: Liang Z 

PROVIDER: S-EPMC2886130 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Molecular hallmarks of anti-chromatin antibodies associated with the lupus susceptibility locus, Sle1.

Liang Zhiyan Z   Chang Sooghee S   Youn Min So MS   Mohan Chandra C  

Molecular immunology 20090624 13


Anti-nuclear antibodies constitute the hallmark of lupus. The NZM2410-derived Sle1 lupus susceptibility interval on murine chromosome 1 breaches tolerance, leading to the emergence of anti-nuclear autoantibodies targeting nucleosomes. However, little is known about the molecular structure of the anti-nucleosome autoantibodies from this genetically simplified mouse model of lupus. In this study, the immunoglobulin heavy chain and light chain sequences of 50 anti-nuclear monoclonal antibodies deri  ...[more]

Similar Datasets

| S-EPMC6857985 | biostudies-literature
| S-EPMC5610905 | biostudies-literature
| S-EPMC2573226 | biostudies-literature
| S-EPMC3466387 | biostudies-literature
| S-EPMC4534470 | biostudies-literature
| S-EPMC5757094 | biostudies-literature
| S-EPMC6111369 | biostudies-literature
| S-EPMC385085 | biostudies-literature
| S-EPMC6382577 | biostudies-literature
| S-EPMC10228156 | biostudies-literature