Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.
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ABSTRACT:
SUBMITTER: Izsvak Z
PROVIDER: S-EPMC2886193 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Publications
Generating knockout rats by transposon mutagenesis in spermatogonial stem cells.
Izsvák Zsuzsanna Z Fröhlich Janine J Grabundzija Ivana I Shirley James R JR Powell Heather M HM Chapman Karen M KM Ivics Zoltán Z Hamra F Kent FK
Nature methods 20100516 6
Disrupting genes in the rat on a genome-wide scale will allow the investigation of many biological processes linked to human health. Here we used transposon-mediated mutagenesis to knock out genes in rat spermatogonial stem cells. Given the capacity of the testis to support spermatogenesis from thousands of transplanted, genetically manipulated spermatogonia, this approach paves a way for high-throughput functional genomic studies in the laboratory rat. ...[more]
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