Dataset Information
Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
Ontology highlight
ABSTRACT: Background
The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP complex. P-TEFb is required for transcription of most class II genes through stimulation of RNA polymerase II elongation and cotranscriptional pre-mRNA processing.Methodology/principal findings
We show here that caffeine prevents UV-induced dissociation of P-TEFb as well as transcription inhibition. The caffeine-effect does not involve PI3-kinase-related protein kinases, because inhibition of phosphatidylinositol 3-kinase family members (ATM, ATR and DNA-PK) neither prevents P-TEFb dissociation nor transcription inhibition. Finally, caffeine prevention of transcription inhibition is independent from DNA damage.Conclusion/significance
Pharmacological prevention of P-TEFb/7SK snRNP dissociation and transcription inhibition following UV-induced DNA damage is correlated.
SUBMITTER: Napolitano G
PROVIDER: S-EPMC2888590 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Publications
Caffeine prevents transcription inhibition and P-TEFb/7SK dissociation following UV-induced DNA damage.
PloS one 20100621 6
<h4>Background</h4>The mechanisms by which DNA damage triggers suppression of transcription of a large number of genes are poorly understood. DNA damage rapidly induces a release of the positive transcription elongation factor b (P-TEFb) from the large inactive multisubunit 7SK snRNP complex. P-TEFb is required for transcription of most class II genes through stimulation of RNA polymerase II elongation and cotranscriptional pre-mRNA processing.<h4>Methodology/principal findings</h4>We show here ...[more]