Ontology highlight
ABSTRACT:
SUBMITTER: Kurata S
PROVIDER: S-EPMC2890720 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Kurata Shinya S Nielsen Klaus H KH Mitchell Sarah F SF Lorsch Jon R JR Kaji Akira A Kaji Hideko H
Proceedings of the National Academy of Sciences of the United States of America 20100601 24
After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC was disassembled by ATP and eukaryotic elongation factor 3 (eEF3). GTP or ITP functioned with less efficiency and adenosine 5gamma'-(beta,gamma-imido)triphosphate did not function at all. The k(cat) of eEF3 was 1.12 min(-1), which is comparable to that ...[more]