Ontology highlight
ABSTRACT:
SUBMITTER: Paulo JA
PROVIDER: S-EPMC2891571 | biostudies-literature | 2009 Apr
REPOSITORIES: biostudies-literature
Paulo Joao A JA Brucker William J WJ Hawrot Edward E
Journal of proteome research 20090401 4
The alpha7 nicotinic acetylcholine receptor (nAChR) is well established as the principal high-affinity alpha-bungarotoxin-binding protein in the mammalian brain. We isolated carbachol-sensitive alpha-bungarotoxin-binding complexes from total mouse brain tissue by affinity immobilization followed by selective elution, and these proteins were fractionated by SDS-PAGE. The proteins in subdivided gel lane segments were tryptically digested, and the resulting peptides were analyzed by standard mass s ...[more]