Ontology highlight
ABSTRACT:
SUBMITTER: Strack RL
PROVIDER: S-EPMC2891888 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Strack Rita L RL Strongin Daniel E DE Mets Laurens L Glick Benjamin S BS Keenan Robert J RJ
Journal of the American Chemical Society 20100601 24
Like GFP, the fluorescent protein DsRed has a chromophore that forms autocatalytically within the folded protein, but the mechanism of DsRed chromophore formation has been unclear. It was proposed that an initial oxidation generates a green chromophore, and that a final oxidation yields the red chromophore. However, this model does not adequately explain why a mature DsRed sample contains a mixture of green and red chromophores. We present evidence that the maturation pathway for DsRed branches ...[more]