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Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.

ABSTRACT: Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.

SUBMITTER: Kovacs AT

PROVIDER: S-EPMC2893497 | biostudies-literature | 2010 Jun

REPOSITORIES: biostudies-literature

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Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans.

Kovács Akos T AT van Hartskamp Mariska M Kuipers Oscar P OP van Kranenburg Richard R

Applied and environmental microbiology 20100416 12

Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression. ...[more]

PMID: 20400555

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