Selective diffusion barriers separate membrane compartments.
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ABSTRACT:
SUBMITTER: Bruckbauer A
PROVIDER: S-EPMC2895375 | biostudies-literature | 2010 Jul
REPOSITORIES: biostudies-literature
Publications
Selective diffusion barriers separate membrane compartments.
Bruckbauer Andreas A Dunne Paul D PD James Peter P Howes Elizabeth E Zhou Dejian D Jones Roy R Klenerman David D
Biophysical journal 20100701 1
We have investigated exchange of molecules between different membrane domains on a highly compartmentalized cell, the spermatozoon. Using Alexa Fluor 555-cholera toxin B-subunit we have observed clustering of preexisting GM1 gangliosides which diffused across the anterior acrosome-equatorial segment interface but did not access the postacrosome. By contrast, single lipid and protein molecules readily exchanged between all three domains, although they diffused more slowly on nearing and crossing ...[more]
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