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Floxin, a resource for genetically engineering mouse ESCs.

ABSTRACT: We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs.

SUBMITTER: Singla V

PROVIDER: S-EPMC2895430 | biostudies-literature | 2010 Jan

REPOSITORIES: biostudies-literature

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Floxin, a resource for genetically engineering mouse ESCs.

Singla Veena V Hunkapiller Julie J Santos Nicole N Seol Allen D AD Norman Andrew R AR Wakenight Paul P Skarnes William C WC Reiter Jeremy F JF

Nature methods 20091206 1

We describe a method for the highly efficient and precise targeted modification of gene trap loci in mouse embryonic stem cells (ESCs). Through the Floxin method, gene trap mutations were reverted and new DNA sequences inserted using Cre recombinase and a shuttle vector, pFloxin. Floxin technology is applicable to the existing collection of 24,149 compatible gene trap cell lines, which should enable high-throughput modification of many genes in mouse ESCs. ...[more]

PMID: 19966808

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