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Novel carbohydrate-binding module identified in a ruminal metagenomic endoglucanase.

ABSTRACT: Endoglucanase C5614-1 comprises a catalytic module (CM) and an X module (XM). The XM showed no significant homology with known carbohydrate-binding modules (CBMs). Recombinant full-length endoglucanase could bind Avicel, whereas the CM could not. The XM could bind various polysaccharides. The results demonstrated that the XM was a new CBM.

SUBMITTER: Duan CJ

PROVIDER: S-EPMC2901718 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Novel carbohydrate-binding module identified in a ruminal metagenomic endoglucanase.

Duan Cheng-Jie CJ Liu Jun-Liang JL Wu Xi X Tang Ji-Liang JL Feng Jia-Xun JX

Applied and environmental microbiology 20100514 14

Endoglucanase C5614-1 comprises a catalytic module (CM) and an X module (XM). The XM showed no significant homology with known carbohydrate-binding modules (CBMs). Recombinant full-length endoglucanase could bind Avicel, whereas the CM could not. The XM could bind various polysaccharides. The results demonstrated that the XM was a new CBM. ...[more]

PMID: 20472722

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