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Searching for mesophilic Thermotogales bacteria: "mesotogas" in the wild.

ABSTRACT: All cultivated Thermotogales are thermophiles or hyperthermophiles. However, optimized 16S rRNA primers successfully amplified Thermotogales sequences from temperate hydrocarbon-impacted sites, mesothermic oil reservoirs, and enrichment cultures incubated at <46 degrees C. We conclude that distinct Thermotogales lineages commonly inhabit low-temperature environments but may be underreported, likely due to "universal" 16S rRNA gene primer bias.

SUBMITTER: Nesbo CL

PROVIDER: S-EPMC2901743 | biostudies-literature | 2010 Jul

REPOSITORIES: biostudies-literature

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Searching for mesophilic Thermotogales bacteria: "mesotogas" in the wild.

Nesbø Camilla L CL Kumaraswamy Rajkumari R Dlutek Marlena M Doolittle W Ford WF Foght Julia J

Applied and environmental microbiology 20100521 14

All cultivated Thermotogales are thermophiles or hyperthermophiles. However, optimized 16S rRNA primers successfully amplified Thermotogales sequences from temperate hydrocarbon-impacted sites, mesothermic oil reservoirs, and enrichment cultures incubated at <46 degrees C. We conclude that distinct Thermotogales lineages commonly inhabit low-temperature environments but may be underreported, likely due to "universal" 16S rRNA gene primer bias. ...[more]

PMID: 20495053

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