Ontology highlight
ABSTRACT:
SUBMITTER: Zhou Y
PROVIDER: S-EPMC2902271 | biostudies-literature | 2010 Jan
REPOSITORIES: biostudies-literature
Zhou Yubin Y Meraner Paul P Kwon Hyoung T HT Machnes Danya D Oh-hora Masatsugu M Zimmer Jochen J Huang Yun Y Stura Antonio A Rao Anjana A Hogan Patrick G PG
Nature structural & molecular biology 20091227 1
Store-operated Ca(2+) entry through the plasma membrane Ca(2+) release-activated Ca(2+) (CRAC) channel in mammalian T cells and mast cells depends on the sensor protein stromal interaction molecule 1 (STIM1) and the channel subunit ORAI1. To study STIM1-ORAI1 signaling in vitro, we have expressed human ORAI1 in a sec6-4 strain of the yeast Saccharomyces cerevisiae and isolated sealed membrane vesicles carrying ORAI1 from the Golgi compartment to the plasma membrane. We show by in vitro Ca(2+) fl ...[more]