Ontology highlight
ABSTRACT:
SUBMITTER: Kilburn D
PROVIDER: S-EPMC2906142 | biostudies-literature | 2010 Jun
REPOSITORIES: biostudies-literature
Kilburn Duncan D Roh Joon Ho JH Guo Liang L Briber Robert M RM Woodson Sarah A SA
Journal of the American Chemical Society 20100601 25
Crowder molecules in solution alter the equilibrium between folded and unfolded states of biological macromolecules. It is therefore critical to account for the influence of these other molecules when describing the folding of RNA inside the cell. Small angle X-ray scattering experiments are reported on a 64 kDa bacterial group I ribozyme in the presence of polyethylene-glycol 1000 (PEG-1000), a molecular crowder with an average molecular weight of 1000 Da. In agreement with expected excluded vo ...[more]